1x citrate buffer (Thermo Fisher)

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Thermo Fisher 1x citrate buffer
1x Citrate Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/1x+citrate+buffer/Triton+X-100/pm41173972-71-7-10
Average 99 stars, based on 1 article reviews

1x citrate buffer - by Bioz Stars, 2026-10

99/100 stars

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Immunofluorescence:

Article Title: Extracellular RNA as a molecular driver and therapeutic target in abdominal aortic aneurysms.
Article Snippet: .. For immunofluorescence, antigen retrieval was performed using 1x citrate buffer (Thermo Fisher Scientific, USA), and tissues were permeabilized in 0.1% Triton X-100 diluted in blocking buffer of 5% bovine serum albumin in PBS. .. Macrophages were stained using a primary rat anti-mouse CD68 antibody (clone FA-11, 1:50 dilution in blocking buffer; Bio-Rad Laboratories Inc., USA), followed by a donkey anti-rat DyLight 650– conjugated secondary antibody (1:200 dilution in blocking buffer; Invitrogen, Thermo Fisher Scientific).

Article Title: Extracellular RNA as a molecular driver and therapeutic target in abdominal aortic aneurysms
Article Snippet: .. For immunofluorescence, antigen retrieval was performed using 1x citrate buffer (Thermo Fisher Scientific, USA), and tissues were permeabilized in 0.1% Triton X-100 diluted in blocking buffer of 5% bovine serum albumin in PBS. .. Macrophages were stained using a primary rat anti-mouse CD68 antibody (clone FA-11, 1:50 dilution in blocking buffer; Bio-Rad Laboratories Inc., USA), followed by a donkey anti-rat DyLight 650–conjugated secondary antibody (1:200 dilution in blocking buffer; Invitrogen, Thermo Fisher Scientific).

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Incubation:

Article Title: FL496, an FL118-derived small molecule, induces growth inhibition, senescence, and apoptosis of malignant pleural mesothelioma (MPM) cells, and exhibits anti-MPM tumor efficacy strikingly superior to the pemetrexed-cisplatin combination
Article Snippet: .. Tissue slide sections were prepared as follows: Deparaffinized tissue sections were rehydrated and incubated in 1X citrate buffer (pH6, Invitrogen Cat #00–5000) for 20 min using a DAKO PT Link. .. Then, with an Autostainer, the following steps and reagents were used for IHC analyses: (i) Incubation in 3% H 2 O 2 for 15 min; (ii) incubation with 10% normal goat serum 10 min (Thermo Fisher #50062Z) 10 min; (iii) incubation with Avidin/Biotin block (Vector Labs Cat#SP-2001); (iv) incubation with individual primary antibodies diluted in 1% BSA for 30 min; (v) incubation with secondary goat anti-rabbit (Vector labs #BA-1000) for 15 min; (vi) incubation with ABC reagents (Vector Labs Cat #PK 6100) for 30 min; (vii) incubation with DAB substrates (Dako Cat #K3467) for 5 min; (viii) counterstained with DAKO Hematoxylin for 20s; and (ix) completing the final coversliped slides.

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1x citrate buffer (Thermo Fisher)

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